DHC-B02 (Burker Turk)

 
 
 

 

Applications : Cell counts in cerebrospinal fluid (CSF), rare cells

 
  - Blood analysis(Hematology): Blood cell counting
  - Cell culture: Cell concentration measurement, Cell viability test
  - Microbiology : Fungal spores counting

 

Outer Size

Width 25mm x Length 75mm x Thickness 1.6mm

Chamber Depth

0.1 mm

Chamber Volume

10 µl

 

Calculation

 

The full grid on a hemocytometer contains 9 squares, each of which is 1 micro meter (mm) square.  Large cells are counted using the four large corner squares (and the middle one).

For a dense suspension of small calls, the four 1/25 sq. mm corners plus the middle square and the central square are used.

It consists of 9 large squares, each measuring 1 x 1 mm, with the depth of chamber of 0.1 mm.  Each square has a total volume of 0.1 mm3 or 10-4cm3.  The central square is divided into 25 small squares with triple lines and four corner squares divided into 16 small squares.

Each large square has a surface area of 1.0mm2, and a chamber depth of 0.1mm.  There are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, that equates to 1/ 0.0001ml = 10,000 = (104).  The volume factor is 10,000.

 

Cells per ml = Average count per square x dilution factor x 10,000







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